Scientist I/II - Therapeutic Genome Editing - Neuromuscular
Brisbane, CA, USA
Posted on Friday, September 1, 2023
Mammoth is currently seeking a talented and detail-oriented Scientist I/II to join our therapeutic genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute translational research studies on mammalian cell lines and primary human cells and in mice to support the development of Cas nucleases for human therapeutics. The position requires adaptability, excellent technical and analytical skills, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment.
- Execution of molecular biology, cloning, cell culture, genome editing, transfection, viral transduction and a broad range of functional assays
- Design and execute experiments to develop, refine, and validate in vitro and in vivo CRISPR systems in muscle in vitro system and tissues
- Apply advanced techniques in molecular biology, cellular biology, and biochemistry to demonstrate CRISPR efficacy and tolerability in muscle in vitro and in vivo systems
- Collaborate closely with cross-functional teams including molecular biologists, bioinformaticians, and biologists to ensure the successful translation of research findings into actionable development strategies
- Provide technical support for phenotyping and analytical characterization of cellular or animal models and genome editing outcomes
- Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members
- Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements
- Other duties as assigned
- Bachelor’s degree in molecular and cell biology, immunology, biochemistry, or a related field and 5 years relevant industry experience or Masters degree and 3 years experience or PhD in molecular and cell biology, immunology, biochemistry, or a related field (Scientist I)
- Bachelor’s degree in molecular and cell biology, immunology, biochemistry, or a related field and 6 years relevant industry experience or Masters degree and 4 years experience or PhD and at least 1 year of industry experience (Scientist II)
- Proficiency in mammalian cell culture, transfections, viral transductions, molecular cloning, PCR, genomic DNA and mRNA purification, cDNA library prep, qPCR, ddPCR, NGS library prep, ELISA, western blot
- 5+ years industry work experience
- Master’s degree
- Experience working with AAV and/or LNP
- Experience with NGS sample preparation
- Experience with complex cell culture systems (cardiomyocytes, myoblasts, myotubes) or patient derived cells (iPSC, fibroblasts)
- Experience developing human therapies using genome editing systems
- Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.)
- Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills.
- Highly motivated with ability to work efficiently and productively in a fast-paced highly collaborative team environment
- Company-paid health/vision/dental benefits
- Unlimited vacation and generous sick time
- Company-sponsored meals and snacks
- Wellness, caregiver and ergonomics benefits
- 401(k) with company matching
Base Salary Range: $118,750 - $147,000
The listed base salary range is for Mammoth employees in the Bay Area. Actual base salary will be determined by geographic work location, relevant professional experience, applicable skills, and internal equity. The base salary range for those working remotely outside of the San Francisco Bay Area may differ and will be determined by industry market data for the role and specific region.
Mammoth Biosciences is a biotechnology company focused on leveraging its proprietary ultracompact CRISPR systems to develop long-term curative therapies, as well as other applications such as decentralized precision diagnostics. Founded by CRISPR pioneer and Nobel laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington, the company’s ultracompact proteins are designed to enable in vivo gene editing in difficult to reach tissues utilizing both nuclease applications and new editing modalities beyond double stranded breaks, including base editing, gene writing, and epigenetic editing. The company is building out its pipeline of potential in vivo gene editing therapeutics and capabilities and has partnerships with leading pharmaceutical and biotechnology companies to broaden the reach of its innovative and proprietary technology. Mammoth’s deep science and industry experience, along with a robust and differentiated intellectual property portfolio, have enabled the company to further its mission to transform the lives of patients and deliver on the full promise of CRISPR technologies.
It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth, and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation.
Mammoth Biosciences requires that all employees be vaccinated against COVID-19 as a condition of at-will employment, with exceptions for medical or religious reasons in compliance with local, state, and federal law.